Abstract: Objective  To explore the value of urine ubiquitin 52 amino acid fusion protein(UbA52) and urine vitamin D binding protein(VDBP) in diabetic nephropathy(DN) diagnosis by detecting urine UbA52 and urine VDBP concentrations.Methods  A total of 46 patients with DN and 38 patients with type 2 diabetes mellitus were treated as  DN  group and diabetic group.ELISA was used to detect UbA52 and VDBP in urine. Analysis of differences between three groups, the differences on DN diagnostic specificity and sensitivity in urine UbA52 and urine VDBP,  urinary protein quantitative and urinary creatinine ratio (ACR) were compared.Results  Urine UbA52 and urine VDBP of  three groups were significantly different from each other(P＜0.05). Urine UbA52 and urine VDBP of DN group were significantly higher than those of diabetes group and normal control group.Urine UbA52 and urine VDBP of DN group and normal control group were not significantly different (P＞0.05).  The area under the ROC curve of  urine UbA52,  urine VDBP，24hour urine protein quantification，ACR，urine UbA52 combined urine VDBP in diagnosis of  DN were 0.729, 0.776，0.629, 0.657，0.779，respectively.Conclusion  The detection of  urine UbA52 and urine VDBP levels have certain reference value in evaluating the condition of  DN. The combined detection of urine UbA52 and urine VDBP has important  clinical significance in  diagnosis of  DN.

Key words: diabetic nephropathies, ubiquitins, vitamin D binding protein; , diagnosis